化學(xué)與生物傳感器是細(xì)胞與活體內(nèi)分子成像的重要工具，但如何在特定的時(shí)間和空間實(shí)現(xiàn)選擇性成像仍然是一個(gè)難題。針對(duì)這一問(wèn)題，國(guó)家納米科學(xué)中心李樂(lè)樂(lè)研究員課題組通過(guò)學(xué)科交叉融合提出了利用上轉(zhuǎn)換發(fā)光技術(shù)遠(yuǎn)程操控化學(xué)與生物傳感的新原理，突破了傳統(tǒng)傳感器設(shè)計(jì)的局限性，實(shí)現(xiàn)了細(xì)胞與活體內(nèi)時(shí)空選擇性分子成像。在該方法中，首先通過(guò)功能性DNA分子設(shè)計(jì)以及在特定位點(diǎn)修飾光敏感鍵，來(lái)抑制探針?lè)肿拥膫鞲泄δ?；進(jìn)而引入上轉(zhuǎn)換納米顆粒與該傳感分子相結(jié)合構(gòu)建智能傳感器件；上轉(zhuǎn)換納米顆粒在近紅外光照射下發(fā)出紫外光，從而選擇性的在特定時(shí)間和空間激活傳感分子的檢測(cè)和成像功能。該工作于2018年發(fā)表于JACS后被JACS Spotlights亮點(diǎn)報(bào)道，入選ACS Editors’ Choice，限時(shí)免費(fèi)開放給讀者。該文章在國(guó)內(nèi)外相關(guān)領(lǐng)域產(chǎn)生了廣泛影響，迄今被引用100余次，并入選ESI高被引論文。美國(guó)科學(xué)院院士Chad A. Mirkin在Adv. Mater. 2020, 32, 1901743發(fā)表綜述文章評(píng)價(jià)：“該方法的優(yōu)勢(shì)在于，利用外界刺激對(duì)傳感器進(jìn)行遠(yuǎn)程控制，從而賦予成像功能在時(shí)間上的可控性”。Nano Today雜志以“Remote-Controlled DNA Nanodevice Only On When Needed”為標(biāo)題對(duì)該工作進(jìn)行了專題報(bào)道（Nano Today 2018, 19, 3）。
This is an elegant study that nicely integrates aptamer technology, upconverting nanoparticle technology and light controlled functional material modification into a powerful intracellular sensing system for the detection of small molecules in cells. The manuscript is well composed and easy to follow. Most experiments were well controlled and the data organization/flow logics are excellent as well.
國(guó)家納米科學(xué)中心
National Center for Nanoscience and Technology
Upconversion Luminescence-Activated DNA Nanodevice for ATP Sensing in Living Cells
2018,?Vol.?140,?No.?2,?578-581
https://pubs.acs.org/doi/10.1021/jacs.7b11161
